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fifty years have ridden off into the sunset
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I'm sorry but I'm just thinking of
the right words to say
I know they don't sound the way
I planned them to be
But if you wait around a while
I'll make you fall for me
I promise, I promise you I will
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| 11:06 AM, Tuesday, June 17, 2003 |
Thought of the moment: running buffer for western blots is weird stuff. It's basically a solution which causes the DNA to shift through the gel by molecular weight when electricity is applied. DNA is a polar molecule so it sorts itself out ... anyway, to load the gels you mark where the DNA is supposed to be inserted. Each well gets an ink mark.
So I look down into the gel chamber and see this black line floating around ... and I realize that it's the ink.
The ink solidified and detached from the gel plate. The running buffer did something really, really strange to that ink. I almost wish I knew what it was, 'cause it was kinda cool.
Unfortunately when I tried to remove the ink from the running buffer it all went to pieces. But it was still cool.
-David
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